ANGI-10. IMPLICATIONS OF EPITHELIAL MEMBRANE PROTEIN-2 (EMP2) IN THE PATHOGENESIS OF MENINGIOMAS

Abstract INTRODUCTION VEGF-A is an important mediator of angiogenesis in meningiomas. However, targeting this protein with bevacizumab has offered only a modest improvement survival, suggesting that there are other compensatory mechanisms involved, enabling vascularization despite ongoing treatment. Epithelial membrane 2 (EMP2), cell surface protein, promotes angiogenic processes gynecological cancers and brain tumors including gliomas glioblastoma. little known about its etiology mechanism action within OBJECTIVE We aimed to evaluate EMP2 expression non-pathologic tissue, tumor specimens, meningioma lines. also understand cells regard proliferation invasion. METHODS Non-pathologic tissues from routine autopsies specimens surgical resection were analyzed. Additionally, four lines obtained (CH157, BEN-MEN1, SF1335 IOMM-LEE) changes cellular invasion measured. Furthermore, was modulated using lentiviral infection. A p-value < 0.05 considered statistically significant. RESULTS mRNA significantly higher the compared meninges. Its positively correlated angiomatous pattern expression. In vitro, high noted all lines, quantification demonstrated highest malignant line, IOMM-LEE. Viral vectors subsequently utilized create overexpressing knockdown IOMM-LEE models. Western analysis correlation between overexpression HIF1α, key growth angiogenesis. CONCLUSION Our data primary samples vitro models implicate pathogenesis. This warrants further investigation by evaluating effects anti-EMP2 therapy on invasiveness both andin vivo